Such assays are suitable for the measurement of proteins at concentrations >5– 10 mg/L. .. Development of an automated immunoturbidimetric ferritin assay. May 3, Different laboratory methods are used to quantify ferritin . assays and automated equipment developments, and in routine laboratory assays and Seco M. Development of an automated immunoturbidimetric ferritin assay. J. Colloid. Interface Sci. (2): – 3. Borque, L., Rus, A., Bellod, L., and Seco, M.L. Development of an automated immunoturbidimetric ferritin assay.
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Development of an automated immunoturbidimetric ferritin assay.
View inline View popup. We suggest an alternative approach that uses a small amount of immunopurified antibody and stabilizes the reagent by saturation of free surface with BSA in a two-step sequential covalent procedure. J Biomater Sci Polym Ed ; 3: J Immunol Methods ; Recent studies of CRP concentrations within the conventional reference interval have suggested new clinical applications.
Ferritin is an frrritin respondent, and so individuals with conditions association with an inflammatory response may have elevated serum ferritin levels that are not directly related iron stores. Clin Chem ; 45 Suppl 6: Clin Chem ; Evaluation of four automated high-sensitivity C-reactive protein methods: The determination of ferritin is useful in diagnosis of defects in iron metabolism, monitoring iron therapy, ascertaining the iron reserves in groups at risk and in the differential diagnosis of anemias.
Carboxilated latex particles nm in diameter were covalently coupled with immunopurified F ab’ 2 fragments of anti-ferritin IgG antibodies. Jadsamount of antibody adsorbed; Jinamount of antibody added.
The quantitative assessment of body iron. The turbidimetric method affords a measuring range immuhoturbidimetric 0. A rapid and sensitive automated light scattering immunoassay for serum C-reactive protein and the definition of a reference range in healthy blood donors.
We chose this microparticle reagent, with a coverage of 0. Principle of Test Method: The effect of varying the particle size on a reagent with a constant charge of 0. The microparticle reagent response of the different antibodies to increasing CRP concentrations can be seen in Fig.
Samples with CRP values above the upper limit of the calibration curve can be reanalyzed after automated or manual sample dilution.
Clin Chem ; 42 Suppl 6: Ann Clin Biochem ; Ceffect of particle size on absorbance at nm. Lancet ; We have developed a new procedure for turbidimetric measurement of ferritin concentration in human serum, based on latex microparticle agglutination technology. Some use expensive monoclonal antibodies, and others use higher sample volumes and antibody concentrations in the reaction mixtures, which can increase deve,opment reactions For practical use, it is essential to obtain microparticle reagents colloidally stable under reaction and storage conditions, with the stability often depending on protein coverage.
The covalently coupled isotherms of IgG anti-CRP on carboxyl-modified polystyrene particles nm diameter are shown in Fig. Recent reports show median values in healthy controls of 0. Variability in the measurement of C-reactive protein in healthy subjects: Among the many commercially available assays for CRP, the most common use fluid-phase or particle-enhanced turbidimetric or nephelometric procedures 7. Covalent isotherm A and dose—response curves as a function asay antibody type and coverage B and particle size C.
Colloidal particles coated with polyclonal IgG usually are not very stable, mainly because of the high antibody coverage needed to obtain good sensitivities 3. Conditions for CRP determination. The procedure has been automated using the Falcor analyzer.
C-reactive protein and bacterial infection in preterm infants. Test Directory Entire Site. Clin Chem ; A microparticle size of nm and a wavelength of nm were selected as the best choice because of the high slope and wide dynamic range showed.
In addition, we present a new procedure of sequential covalent coupling of IgG and bovine serum albumin BSA that improves the reagent colloidal stability and could eliminate most of the drawbacks of the light-scattering immunoassays, such as nonspecific agglutination.
It allows lower antibody coverage on the microparticle reagent and provides highly sensitive and robust particle-enhanced turbidimetric immunoassay for serum CRP.
Parameter values abcorrelation coefficients rand confidence assah were as follows: A new method of measuring C-reactive protein, with a low limit of detection, suitable for risk assessment of coronary heart disease.
The ferritin assay is an automated immunoturbidimetric method. Ferritkn immunopurified antibody showed a similar trend, having a covalently coupled adsorption isotherm according to that of the nonimmunopurified antibody in the range studied 0. J Clin Immunoassay ; For the immunopurified anti-CRP antibodies, we used a modified procedure consisting of two sequential covalent coupling steps.
Clinical Chemistry Nov46 11 .